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M9490643.TXT
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1994-09-24
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Document 0643
DOCN M9490643
TI Identification of astrovirus serotypes from children treated at the
Hospitals for Sick Children, London 1981-93.
DT 9411
AU Noel J; Cubitt D; Department of Virology, Hospital for Sick Children,
London.
SO Epidemiol Infect. 1994 Aug;113(1):153-9. Unique Identifier : AIDSLINE
MED/94341312
AB An enzyme immunoassay (EIA) for astrovirus type 1 together with immune
electronmicroscopy (IEM) was used to type a collection of 162
astroviruses obtained from 1981-93 from children with diarrhoea. The EIA
was found to be specific for astrovirus type 1. Astrovirus types 2-4
were typed by IEM. Astrovirus type 1 was the prevalent serotype 107/125
(86%), followed by type 3 (8%), type 4 (6%) and type 2 (1%). Six samples
containing astrovirus could not be typed or detected by EIA because they
were coated with coproantibodies; 11 others were not identified. Virus
particles could no longer be detected in 15/162 (9%) samples following
storage for > or = 2 years. Selected samples containing astrovirus types
1-4 were passaged in CaCO2 cells and their identity confirmed by one or
both assays. One sample was shown to have remained viable for 10 years
when stored as an aqueous suspension at -20 degrees C. Two patients with
severe combined immune deficiency disease (SCID) were shown to be
excreting astrovirus type 1 for 32 and 102 days respectively. One child
was simultaneously shedding rotavirus and the other child was excreting
adenovirus.
DE Astroviruses/*CLASSIFICATION/ULTRASTRUCTURE Child
Diarrhea/COMPLICATIONS/*MICROBIOLOGY Human Immunoenzyme Techniques
London Microscopy, Electron Microscopy, Immunoelectron Serotyping
Severe Combined Immunodeficiency/COMPLICATIONS Virus
Diseases/COMPLICATIONS/*MICROBIOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).